cd86 rabbit pab  (Bioss)

Journal: Materials Today Bio

Article Title: A macrophage-like biomimetic nanoparticle with high-efficiency biofilm disruption and innate immunity activation for implant-related infection therapy

doi: 10.1016/j.mtbio.2025.101575

Figure Lengend Snippet: Immunomodulatory effects of F/R@PM on macrophages in vitro. ( A ) Typical scatter plots of macrophage surface markers CD86 (M1 macrophage marker) as detected using a flow cytometer. ( B ) Representative western blot results of the expression of CD86 protein after various treatments. ( C ) Corresponding quantitative analyses of western blot results (n = 3, ∗∗ P < 0.01). ( D ) ELISA results of cytokines (TNF- α and IL-6) secreted by RAW264.7 in different groups (n = 3, ∗∗ P < 0.01). ( E ) Counted results of phagocytized S. aureus by RAW264.7 treated in different conditions (n = 3, ∗∗ P < 0.01). ( F ) Swallowed S. aureus was observed by using TEM. Yellow arrows indicate S. aureus . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: For IHC analysis, tissue slices were incubated with the primary antibodies anti-CD86 Rabbit pAb (1:100, Bioss), TNF-α Rabbit pAb (1:100, Servicebio), and IL-6 Rabbit pAb (1:100, Servicebio) for 12 h, followed by secondary antibody HRP-conjugated goat anti-rabbit IgG ([H + L], 1:100, Servicebio) for 0.5 h. The diaminobenzidine detection kit was used to visualize the binding sites.

Techniques: In Vitro, Marker, Flow Cytometry, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Materials Today Bio

Article Title: A macrophage-like biomimetic nanoparticle with high-efficiency biofilm disruption and innate immunity activation for implant-related infection therapy

doi: 10.1016/j.mtbio.2025.101575

Figure Lengend Snippet: Immunomodulatory function of F/R@PM in vivo. ( A ) Immunohistochemical images of peripheral tissues stained with CD86 antibody. ( B ) Immunohistochemical images of peripheral tissues stained with TNF- α and IL-6 antibody. ( C ) ELISA results of peripheral tissue homogenates. (n = 3, ∗∗ P < 0.01).

Article Snippet: For IHC analysis, tissue slices were incubated with the primary antibodies anti-CD86 Rabbit pAb (1:100, Bioss), TNF-α Rabbit pAb (1:100, Servicebio), and IL-6 Rabbit pAb (1:100, Servicebio) for 12 h, followed by secondary antibody HRP-conjugated goat anti-rabbit IgG ([H + L], 1:100, Servicebio) for 0.5 h. The diaminobenzidine detection kit was used to visualize the binding sites.

Techniques: In Vivo, Immunohistochemical staining, Staining, Enzyme-linked Immunosorbent Assay